The nucleotide sequence of Scenedesmus obliquus chloroplast elongator methionine-accepting tRNA
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چکیده
منابع مشابه
The nucleotide sequence of 5S rRNA from Scenedesmus obliquus.
The nucleotide sequence of the cytoplasmic 5S ribosomal RNA from Scenedesmusobliquus has been determined using post-labelling techniques. The sequence is closely related to the 5S RNA sequence of Chlorella and contains all the areas of invariant sequence which appear to be conserved in plant cytoplasmic 5S RNA species.
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Chloroplast-free mutant cells C-2A ' of the green algae Scenedesmus obliquus lack thioredoxin / , which functions in the light activation of chloroplast enzymes, but do con tain the regular thioredoxins I and II. When dark-grown algae are transferred to light, thioredoxin / activity appears rapidly and increases in parallel with photosynthetic ac tivities; however it precedes chlorophyll bios...
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Clones including tRNA genes from tomato {Lycopersicon esculentum) chloroplast DNA have been obtained. Here we report the nucleotide sequence of tmH\ trnD-^ and tmL\ Sequences were determined by the dideoxy method (1). In higher plant chloroplast genomes, tmY and trnD are clustered together with trnE in the large single copy (LSQ region (2). The sequence of trnY reveals 100% identity with its co...
متن کاملYeast mitochondrial methionine initiator tRNA: characterization and nucleotide sequence.
Two methionine tRNAs from yeast mitochondria have been purified. The mitochondrial initiator tRNA has been identified by formylation using a mitochondrial enzyme extract. E. coli transformylase however, does not formylate the yeast mitochondrial initiator tRNA. The sequence was determined using both 32P-in vivo labeled and 32P-end labeled mt tRNAf(Met). This tRNA, unlike N. crassa mitochondrial...
متن کاملTransfer ribonucleic acid from Scenedesmus obliquus D3.
Scenedesmus obliquus is a green alga whose cells have a well-defined nucleus, and contain a chloroplast. Even when grown in the dark these cells retain their chloroplast. Because of the toughness of the cell wall, extraction of tRNA requires either vigorous homogenization of the cells before phenol extraction or prolonged phenol extraction at 60°C. Determination of the amount of tRNA and its pu...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2380297